ct3d/at3d Cell Tracking

The ct3d/at3d Cell Tracking Tools

Contents

About
Download
Installation
Getting Started

Introduction

ct3d is a command-line tool for tracking cells in time series of 2D images or 3D image stacks. ct3d performs the basic cell tracking as a command line tool. Visualization, exploration, and evalutation of the results can be done through the graphical user interface of at3d.

Download

ct3d at3d

Installation

Source Code Version

ct3d requires libtiff as well as lpsolve Version 5.5. at3d requires libtiff and qt. Make sure all these packages are properly installed. After downloading the source code versions of ct3d and at3d, type make in the corresponding paths to build the program.

Binary Versions

Binary versions are currently available for Linux (64 bit) and MacOS.

Linux: Make sure you have all required libraries properly installed (libtiff as well as lpsolve Version 5.5 for ct3d, as well as libtiff and qt for at3d). Download and unpack the files linked above, and launch the programs AT3D and CT3D from the command line. Note that all binaries are compiled for 64 bit systems only.

MacOS: For launching ct3d, make sure that both libtiff as well as lpsolve Version 5.5 are properly installed, and your DYLD_LIBRARY_PATH points to the respective .dyld files of these libraries. The binary version of at3d includes all dependant libraries. Downlaod and unpack the compressed archive file. at3d can be launched by clicking AT3D.app in the finder, or typing open AT3D.app in a shell window.

Getting Started

For a brief introduction including a sample dataset, refer to the Getting Started page.

License

ct3d is published under the GPLv3.

Acknowledgments

ct3d uses Emil Stefanov's implementation of the disjoint sets data structure for constructing component trees.

ct3d/at3d Getting Started
Hang Xiao and Axel Mosig, Pattern Discovery Group, CAS-MPG Partner Institute for Computational Biology, Shanghai, China